Single-cell RNA-Seq

The results of the scRNA-Seq can be explored through the web app:

CeNGENApp to explore the scRNA-Seq dataset

Help and documentation:

Extensive documentation of the website functionalities
Common use cases Q&A
Data Q&A

You can also download a local version following the instructions here.

We have performed 10x single-cell RNA-Seq on FACS-isolated neurons. Using 100,955 sequenced cells from 17 experiments, 128 neuronal classes have been identified and computationally assigned to a cluster based on their gene expression fingerprint.

We additionally validated the neuronal classes identified by creating novel expression markers, and compared our results to bulk-RNA-Seq on sorted neurons from 8 classes. We performed several additional analyses, described in the associated paper and preprint.

The raw sequencing results are available on GEO (GSE136049), we also created a user-friendly Web interface to interrogate the dataset (see above). More advanced users who wish to download a local version of the Web app can follow the instructions here.