Single-cell RNA-Seq

We have performed 10x single-cell RNA-Seq on FACS-isolated neurons. Using 100,955 sequenced cells from 17 experiments, 128 neuronal classes have been identified and computationally assigned to a cluster based on their gene expression fingerprint.

We additionally validated the neuronal classes identified by creating novel expression markers, and compared our results to bulk-RNA-Seq on sorted neurons from 8 classes. We performed several analyses, described in the associated preprint. We created a user-friendly Web interface to interrogate the dataset. The raw sequencing results are available on GEO (GSE136049).