Advancement of bulk sequencing

Genetic approach: we have validated markers for 55/121 neuronal classes, with 56 others under construction. We are still looking for a valid approach for these classes: AIB, AQR, PQR, CEP, BDU, M2, M3, M5, MC, MI, PDB, PLN, PVN, PVP, and URX. Suggestions are welcome!

Sorting: at least 4 independent samples have been sorted for 49/119 neuronal classes.

Sequencing: at least 3 samples have been sequenced for 47 neuron classes.

Data release: data for 8 neuron classes has been released (see the accession number in the table).

Neuronal
class
Validated genetic approachSortingSequencingData release
AWAPY10421 [gpa-4p(d6)::myrGFP]donein progressGSE169137
LUAXE2299 pha-1(e2123); wpEx389 [C39H7.2::NLS GFP + pha-1]donein progress
PHAOH16065_otls747 [srg-13p::gfp]; otls355 [rab-3p::2xNLS::TagRFP]donein progress
PHBin progress
RMFin progress
RMHin progress
SAAin progress
SIBin progress
ASHin progress
RMEdone
PDEin progress
ALMin progress
AVMgcy-35::GFP; mec-3::RFPdonein progress
FLPTU5066
mec-3p::RFP; sto-5p::GFP
in progress
PLMin progress
PVMTU6276
uIs115 [mec-17p::RFP]; kuIs70 [alr-1::GFP; rol-6(D)]
donein progress
SDQin progress
RIVin progress
RMD DVOH16077 pha-1 (e2123) otEx7419 [mgl-1 p::gfp,pha-1 (+)]donein progress
SMDOH15507
otIs439 [lad-2::GFP]; hdIs32 [glr-1::dsRED2]
donein progress
AINOH16151 lgc-35::tagRFP; ynIs34[flp-19::GFP]; pha-1donein progress
ASin progress
AVBin progress
DBin progress
DVAin progress
PDAin progress
PVCOH16749 hdIs32[glr-1::DsRed2]; ynIs54[flp-20p::GFP]donein progress
RIMOH16917donein progress
VA-
VBbnc-1::GFP; ufIs26 [unc-4::mCh]donein progress
AVDin progress
AVENC1750 KM173
opt-3::GFP[pRF4];
hdIs32 [glr-1::DsRed2]
donein progressGSE169137
AVANC1749 OH4326
rig-3::GFP; pha-1(+); hdIs32 [glr-1::DsRed2]
donein progress
DVCNC3540 otIs458 [ceh-63p::GFP, unc-119(+)]donein progress
PHCin progress
AFDoyls17 [gcy-8p::GFP, lin-15(+)]donein progress
AIB-
ASELOH3191
otIs3 [gcy-7::GFP + lin-15(+)]
donein progress
ASERVH804
ntls1 [gcy-5p::GFP]
donein progress
AWCOH16052 otls745 [ceh-36p3::npp-9::mCherry::BLRP·:3x FLAG::npp-9 3'UTR]donein progress
CEPin progress
ASGOH14973
pha-1(e2123); him-5(e1490); otEx6966 [srw-119prom::GFP; pha-1(+)]
donein progressGSE169137
AVGZM9592 hpIs670 [pnmr-1::GFP ZF; pglr-5::ZIF-1::SL2::wCherry; lin-15(+)]donein progressGSE169137
PVPin progress
RIAIK705
njls10 [glr-3::GFP]
donein progress
ADFPY3470
oyIs51 [srh-142::RFP]; myIs13[klp-6p::GFP]
donein progress
ASJin progress
AIMotIs520 [eat-4::prom11] (also in PHC)donein progress
AIZin progress
ALAXE2459 [flp-13:mcherry]; pha-1; ynIs66 [flp-7p::GFP]-
AQRin progress
AUAin progress
AVFin progress
AVLNY2051 ynIs51 [Pflp-22::GFP] ,[flp-22p::GFP]; him-5(e1490)donein progress
CANF49C12.10::GFPdonein progress
PVQOH16952donein progress
PVTin progress
RIDin progress
RIFin progress
RIGin progress
RMGdone
URBin progress
URXin progress
URYin progress
VCNC3524
ufIs26 [punc-4::mCherry]; vsIs13 [lin-11::pes-10::GFP; lin-15(+)]
donein progress
AIAin progress
BDUin progress
HSNOH15925 otIs737[otEx7359[cat-4p::mCherry, rol-6]]
IL2myls13 [klp-6p::GFP, pha-1(+)]; oyIs51 [srh-142::RFP]donein progress
URA-
IL1NY2021
ynls21 [flp-3p::GFP, rol-6]
donein progress
ASKOH15857
[srg-8p::gfp; inx-6p18::tagRFP]
donein progress
DVBin progress
ADLOH16093 pha-1 (e2123) otEx6853 [srv-3p:: gfp; pha-1 (+); otls356[rab-3p::2xNLS::TagRFP]donein progress
ASIFK181
ksIs2 [daf-7p::GFP, rol-6(su10060)]
donein progress
PVRin progress
I1in progress
I2in progress
I3in progress
I4in progress
I5ynIs37[flp-13::gfp]; ufIs26[unc-4::mCherry]donein progress
I6in progress
M1-
M2in progress
M3in progress
M4OH16144 ceh-28p::4NLS::GFP donein progress
M5in progress
MCin progress
MIin progress
NSMLX837
[tph-1::GFP]
donein progress
OLQdone
RIBin progress
RIP-
RIRin progress
RIHin progress
SABin progress
SIAin progress
SMBOH16781 otIs796 X[flp-12DEL3::gfp; unc-122p::Red]donein progress
ADA-
ADE-
AIYOH99
mgIs18 [ttx-3p::GFP]
donein progress
ALNin progress
AVHOH16483 [unc-119(ed3); otIs768 [hlh-34p::gfp, unc-119(+)]]donein progress
AVJin progress
AVKMU1082
bwIs2[flp-1::gfp, rol-6]
donein progress
AWBCX3553
lin-15(n765); kyls104 [str-1p::GFP, lin-15(+)]
donein progressGSE169137
BAGRJP567
ynls64 [flp-17p::GFP]
donein progress
DANC2913
hdIs1 [unc-53::GFP rol-6(su1000)]; ufIs26 [unc-4::mCherry]
donein progress
DDNC3296
juIs223[pttr-39::mCherry; pttx-3::GFP]; ynIs37[flp-13::GFP]
donein progressGSE169137
OLLOH16719 otIs138 [ser-2p3 + rol-6(su1006)] X; otIs521[eat-4prom8::tagRFP + ttx-3::GFP]donein progress
PDB-
PLN-
PQRin progress
PVDNC3182
otIs138[ser-2prom3::GFP + rol-6]; otIs396[ace-1prom2::NLS::TagRFP]; otIs181[dat-1::mCherry]
donein progressGSE169137
PVNin progress
PVW-
RICOH16150 nIs107(tbh-1::gfp) III; zfIs10(tdc-1::m-cherry) IVdonein progress
RISHBR1261
goeIs500 [flp-11prom::mKate; unc-119(+)]
donein progress
RISNC2015
ynIs40 [flp-11::GFP]; hdIs32 [glr-1::DsRed2]
donein progress
VDNC3296
juIs223 [pttr-39::mCherry; pttx-3::GFP]; ynIs37 [flp-13::GFP]
donein progressGSE169137
RMD LRin progress