Advancement of bulk sequencing

Genetic approach: we have available markers for 33/119 neuronal classes, with 72 others under construction. We are still looking for a valid approach for these 1 classes: ADA, ADE, M1, PDB, PLN, PVW, RIP, URA, URB, URY, VA. Suggestions are welcome!

Sorting: at least 4 independent samples have been sorted for 36/119 neuronal classes.

Sequencing: at least 3 samples have been sequenced for 19 neuron classes.

Data release: data for 8 neuron classes will be released shortly.

Neuronal
class
Validated genetic approachSortingSequencingData release
AWAPY10421 [gpa-4p(d6)::myrGFP]donein progressupcoming
LUAXE2299 pha-1(e2123); wpEx389 [C39H7.2::NLS GFP + pha-1]done
PHAOH16065_otls747 [srg-13p::gfp]; otls355 [rab-3p::2xNLS::TagRFP]in progress
PHBin progress
RMFin progress
RMHin progress
SAAin progress
SIBin progress
ASHin progress
RMEin progress
PDEin progress
ALMin progress
AVMin progress
FLPTU5066
mec-3p::RFP; sto-5p::GFP
in progress
PLMin progress
PVMTU6276
uIs115 [mec-17p::RFP]; kuIs70 [alr-1::GFP; rol-6(D)]
done
SDQin progress
RIVin progress
RMDOH16077 pha-1 (e2123) otEx7419 [mgl-1 p::gfp,pha-1 (+)]in progress
SMDOH15507
otIs439 [lad-2::GFP]; hdIs32 [glr-1::dsRED2]
done
AINOH16151 lgc-35::tagRFP; ynIs34[flp-19::GFP]; pha-1in progress
ASin progress
AVBin progress
DBin progress
DVAin progress
PDAin progress
PVCin progress
RIMin progress
VA-
VBbnc-1::GFP; ufIs26 [unc-4::mCh]donein progress
AVDin progress
AVENC1750 KM173
opt-3::GFP[pRF4];
hdIs32 [glr-1::DsRed2]
donein progressupcoming
AVANC1749 OH4326
rig-3::GFP; pha-1(+); hdIs32 [glr-1::DsRed2]
donein progress
DVCNC3540 otIs458 [ceh-63p::GFP, unc-119(+)]done
PHCin progress
AFDoyls17 [gcy-8p::GFP, lin-15(+)]donein progress
AIBin progress
ASELOH3191
otIs3 [gcy-7::GFP + lin-15(+)]
done
ASERVH804
ntls1 [gcy-5p::GFP]
done
AWCOH16052 otls745 [ceh-36p3::npp-9::mCherry::BLRP·:3x FLAG::npp-9 3'UTR]done
CEPin progress
ASGOH14973
pha-1(e2123); him-5(e1490); otEx6966 [srw-119prom::GFP; pha-1(+)]
donein progressupcoming
AVGZM9592 hpIs670 [pnmr-1::GFP ZF; pglr-5::ZIF-1::SL2::wCherry; lin-15(+)]donein progressupcoming
PVPin progress
RIAIK705
njls10 [glr-3::GFP]
donein progress
ADFPY3470
oyIs51 [srh-142::RFP]; myIs13[klp-6p::GFP]
donein progress
ASJin progress
AWAPY10421
[gpa-4p(d6)::myrGFP]
done
AIMin progress
AIZin progress
ALAXE2459 [flp-13:mcherry]; pha-1; ynIs66 [flp-7p::GFP]in progress
AQRin progress
AUAin progress
AVFin progress
AVLin progress
CANF49C12.10::GFPdonein progress
PVQin progress
PVTin progress
RIDin progress
RIFin progress
RIGin progress
RMGin progress
URB-
URXin progress
URY-
VCNC3524
ufIs26 [punc-4::mCherry]; vsIs13 [lin-11::pes-10::GFP; lin-15(+)]
done
AIAin progress
BDUin progress
HSNin progress
IL2myls13 [klp-6p::GFP, pha-1(+)]; oyIs51 [srh-142::RFP]donein progress
URA-
IL1NY2021
ynls21 [flp-3p::GFP, rol-6]
done
ASKOH15857
[srg-8p::gfp; inx-6p18::tagRFP]
donein progress
DVBin progress
ADLOH16093 pha-1 (e2123) otEx6853 [srv-3p:: gfp; pha-1 (+); otls356[rab-3p::2xNLS::TagRFP]in progress
ASIFK181
ksIs2 [daf-7p::GFP, rol-6(su10060)]
done
PVRin progress
I1in progress
I2in progress
I3in progress
I4in progress
I5in progress
I6in progress
M1-
M2in progress
M3in progress
M4OH16144 ceh-28p::4NLS::GFP done
M5in progress
MCin progress
MIin progress
NSMLX837
[tph-1::GFP]
donein progress
OLQin progress
RIBin progress
RIP-
RIRin progress
RIHin progress
SABin progress
SIAin progress
SMBin progress
ADA-
ADE-
AIYOH99
mgIs18 [ttx-3p::GFP]
donein progress
ALNin progress
AVHin progress
AVJin progress
AVKMU1082
bwIs2[flp-1::gfp, rol-6]
done
AWBCX3553
lin-15(n765); kyls104 [str-1p::GFP, lin-15(+)]
donein progressupcoming
BAGRJP567
ynls64 [flp-17p::GFP]
done
DANC2913
hdIs1 [unc-53::GFP rol-6(su1000)]; ufIs26 [unc-4::mCherry]
donein progress
DDNC3296
juIs223[pttr-39::mCherry; pttx-3::GFP]; ynIs37[flp-13::GFP]
donein progressupcoming
OLLin progress
PDB-
PLN-
PQRin progress
PVDNC3182
otIs138[ser-2prom3::GFP + rol-6]; otIs396[ace-1prom2::NLS::TagRFP]; otIs181[dat-1::mCherry]
donein progressupcoming
PVNin progress
PVW-
RICOH16150 nIs107(tbh-1::gfp) III; zfIs10(tdc-1::m-cherry) IVdone
RISHBR1261
goeIs500 [flp-11prom::mKate; unc-119(+)]
done
RISNC2015
ynIs40 [flp-11::GFP]; hdIs32 [glr-1::DsRed2]
done
VDNC3296
juIs223 [pttr-39::mCherry; pttx-3::GFP]; ynIs37 [flp-13::GFP]
donein progressupcoming