The results of the scRNA-Seq can be explored through the web app:
Help and documentation:
- Extensive documentation of the website functionalities (link, download)
- Common use cases Q&A (link, download)
- Data Q&A (link, download)
You can also download a local version following the instructions here.
We have performed 10x single-cell RNA-Seq on FACS-isolated neurons. Using 100,955 sequenced cells from 17 experiments, 128 neuronal classes have been identified and computationally assigned to a cluster based on their gene expression fingerprint.
We additionally validated the neuronal classes identified by creating novel expression markers, and compared our results to bulk-RNA-Seq on sorted neurons from 8 classes. We performed several additional analyses, described in the associated preprint.
The raw sequencing results are available on GEO (GSE136049), we also created a user-friendly Web interface to interrogate the dataset (see above). More advanced users who wish to download a local version of the Web app can follow the instructions here.