The CeNGEN project

First Data Release! Single-cell RNA-Seq data are now available to explore at https://cengen.shinyapps.io/SCeNGEA

We are pleased to release the first round of single cell RNA-Seq data in our web application SCeNGEA. The data can be downloaded as an R object in the monocle CellDataSet format on the downloads page. For detailed methods and initial description of the data, see our preprint on bioRxiv, https://www.biorxiv.org/content/10.1101/737577v2.

Differential gene expression defines individual neuron types and determines how each contributes to circuit physiology and responds to injury and disease. The C. elegans Neuronal Gene Expression Map & Network (CeNGEN) will establish a comprehensive gene expression atlas of an entire nervous system at single-neuron resolution.

Two approaches are being used:

1) single-cell RNA-Sequencing, which allows the identification of each neuronal class;

2) deep bulk RNA sequencing of sorted neurons from each class enables full characterization of its transcriptome and of non-coding RNAs.

You can find a full description in Hammarlund, Marc et al., Neuron, Volume 99, Issue 3 430-433, doi:10.1016/j.neuron.2018.07.042

This project was made possible by the support of the NIH NINDS (grant R01NS100547) and the C. elegans research community.